Table I.
Conserved spore proteins of B. anthracis and closely related strains selected by genome-based in silico analysis showing reaction with rabbit immune sera
Results were derived from subtraction of control serum (unimmunized, 0.005 at A405) from anti-GerH whole spores and anti-exosporium sera. −, <0.1; +, 0.1–0.2; ++, 0.2–0.3; +++, >0.3. PTS, phosphotransferase system.
| Genes selected | ORFs | Molecular mass | ELISA with rabbit immune sera |
|
|---|---|---|---|---|
| Anti-spore | Anti-exosporium | |||
| bp | kDa | |||
| Sporulation | ||||
| gi47505340; GBAAT34016.1; small, acid-soluble spore protein B | 198 | 7.3 | − | ++ |
| gi47504727; GBAAT33403.1; stage V sporulation protein AF | 1,473 | 54.0 | + | − |
| gi47504853; GBAAT33529.1; stage III sporulation protein AG | 663 | 24.3 | ++ | + |
| gi47505104; GBAAT33780.1; forespore-specific protein, putative | 627 | 23.0 | + | − |
| gi47504730; GBAAT33406.1; stage V sporulation protein AD | 1,017 | 37.3 | + | + |
| gi47505076; GBAAT33752.1; prespore-specific transcriptional regulator rsfA, putative | 642 | 23.5 | + | − |
| gi47505119; GBAAT33795.1; spo0B-associated GTP-binding protein | 1,287 | 47.2 | + | − |
| gi47503187; GBAAT31863.1; spore cortex lytic enzyme prepeptide | 762 | 27.9 | + | − |
| gi47501241; GBAAT29917.1; cotJB protein | 276 | 10.1 | − | ++ |
| Cell division | ||||
| gi47500466; GBAAT29142.1; cell division protein FtsH | 1,902 | 69.7 | +++ | + |
| gi47504423; GBAAT33099.1; signal recognition particle-docking protein FtsY | 990 | 36.3 | + | − |
| gi47504476; GBAAT33152.1; cell division initiation protein DivIVA | 507 | 18.6 | + | − |
| gi47504487; GBAAT33163.1; cell division protein FtsA | 1,302 | 47.7 | ++ | − |
| gi47504408; GBAAT33084.1; gid protein | 1,305 | 47.9 | − | ++ |
| ABC transporter | ||||
| gi47500766; GBAAT29442.1; iron compound-binding protein ABC transporter | 918 | 33.7 | +++ | + |
| gi47505671; GBAAT34347.1; ABC transporter, ATP-binding protein | 786 | 28.8 | − | + |
| gi47505676; GBAAT34352.1; ABC transporter, ATP-binding protein | 1,026 | 37.6 | + | − |
| gi47500801; GBAAT29477.1; ABC transporter, substrate-binding protein, putative | 999 | 36.6 | ++ | − |
| gi47551555; GBAAT29896.2; Na/Pi-cotransporter family protein | 1,656 | 60.7 | − | + |
| gi47501472; GBAAT30148.1; ABC transporter, ATP-binding protein EcsA | 744 | 27.3 | + | + |
| gi47501606; GBAAT30282.1; oligopeptide ABC transporter, ATP-binding protein | 1,044 | 38.3 | − | ++++ |
| gi47501607; GBAAT30283.1; oligopeptide ABC transporter, ATP-binding protein | 936 | 34.3 | + | ++ |
| gi47501710; GBAAT30386.1; spermidine/putrescine ABC transporter, ATP-binding protein | 996 | 36.5 | + | − |
| gi47501714; GBAAT30390.1; spermidine/putrescine-binding ABC transporter protein | 1,038 | 38.1 | + | + |
| gi47502979; GBAAT31655.1; ABC transporter, ATP-binding protein | 669 | 24.5 | + | − |
| gi47503226; GBAAT31902.1; glycine betaine/l-proline ABC transporter, permease protein | 837 | 30.7 | ++ | + |
| Hypothetical | ||||
| gi47502779; GBAAT31455.1; hypothetical protein | 537 | 19.7 | ++++ | ++++ |
| gi47505416; GBAAT34092.1; hypothetical protein | 420 | 15.4 | + | − |
| gi47501650; GBAAT30326.1; hypothetical protein | 504 | 18.5 | ++ | + |
| gi47501749; GBAAT30425.1; hypothetical protein | 342 | 12.5 | + | + |
| gi47504707; GBAAT33383.1; hypothetical protein | 363 | 13.3 | ++ | + |
| Conserved hypothetical | ||||
| gi47506115; GBAAT34791.1; conserved hypothetical protein | 438 | 16.1 | − | + |
| gi47501825; GBAAT30501.1; conserved hypothetical protein | 555 | 20.4 | + | + |
| gi47502061; GBAAT30737.1; conserved hypothetical protein | 249 | 9.1 | + | + |
| gi47551733; GBAAT31416.2; conserved hypothetical protein | 756 | 27.7 | − | +++ |
| gi47551862; GBAAT32537.2; conserved hypothetical protein | 1,062 | 38.9 | ++++ | +++ |
| gi47504112; GBAAT32788.1; conserved domain protein | 264 | 9.7 | + | +++ |
| gi47504632; GBAAT33308.1; conserved hypothetical protein | 213 | 7.8 | ++ | − |
| gi47503999; GBAAT32675.1; conserved hypothetical protein | 1,467 | 53.8 | + | − |
| gi47504358; GBAAT33034.1; conserved hypothetical protein | 912 | 33.4 | + | − |
| gi47504360; GBAAT33036.1; conserved hypothetical protein | 249 | 9.1 | ++ | − |
| gi47504363; GBAAT33039.1; conserved hypothetical protein | 1,275 | 46.8 | + | − |
| gi47504372; GBAAT33048.1; conserved hypothetical protein | 750 | 27.5 | + | − |
| gi47504390; GBAAT33066.1; conserved hypothetical protein | 273 | 10.0 | + | − |
| gi47501585; GBAAT30261.1; conserved hypothetical protein | 198 | 7.3 | − | ++ |
| Lipoprotein | ||||
| gi47505654; GBAAT34330.1; lipoprotein, putative | 576 | 21.1 | +++ | + |
| gi47500656; GBAAT29332.1; lipoprotein, putative | 1,014 | 37.2 | ++ | − |
| gi47503185; GBAAT31861.1; lipoprotein, putative | 597 | 21.9 | + | − |
| gi47502474; GBAAT31150.1; adhesion lipoprotein | 951 | 34.9 | ++ | − |
| Spore germination | ||||
| gi47500552; GBAAT29228.1; spore germination protein GerD | 618 | 22.7 | ++ | − |
| gi47502385; GBAAT31061.1; bacterial luciferase family protein | 1,056 | 38.7 | + | − |
| DNA binding | ||||
| gi47504294; GBAAT32970.1; DNA-binding protein HU | 273 | 10.0 | + | ++ |
| gi47500486; GBAAT29162.1; DNA-binding protein, putative | 1,074 | 39.4 | + | − |
| Amino acid binding | ||||
| gi47552115; GBAAT34692.2; ATP synthase F1, γ subunit | 861 | 31.6 | − | + |
| gi47504102; GBAAT32778.1; thioredoxin-like protein | 198 | 7.3 | + | +++ |
| gi47504630; GBAAT33306.1; polypeptide deformylase | 555 | 20.4 | + | +++ |
| Energy metabolism | ||||
| gi47552124; GBAAT35466.1; 4-oxalocrotonate tautomerase | 186 | 6.8 | − | + |
| gi47504281; GBAAT32957.1; PTS system, fructose-specific IIABC component | 1,881 | 69.0 | + | − |
| gi47504349; GBAAT33025.1; pyruvate ferredoxin oxidoreductase, α subunit, putative | 1,758 | 64.5 | + | − |
| Cell wall | ||||
| gi47551930; GBAAT33049.2; metallo-β-lactamase family protein | 1,671 | 61.3 | + | − |
| gi47503556; GBAAT32232.1; metallo-β-lactamase family protein | 846 | 31.0 | +++ | − |
| gi47500657; GBAAT29333.1; alanine racemase | 1,170 | 42.9 | − | ++ |
| gi47502504; GBAAT31180.1; chaperone CsaA | 330 | 12.1 | − | + |
| Protein degradation | ||||
| gi47504362; GBAAT33038.1; zinc protease, insulinase family | 1,287 | 47.2 | + | − |
| gi47504380; GBAAT33056.1; zinc protease, insulinase family | 1,242 | 45.5 | + | − |
| gi47504406; GBAAT33082.1; ATP-dependent protease hsIV | 543 | 19.9 | + | − |
| gi47504350; GBAAT33026.1; renal dipeptidase family protein | 924 | 33.9 | + | − |
| Cellular process | ||||
| gi47504391; GBAAT33067.1; N-utilization substance protein A | 1,107 | 40.6 | + | − |
| gi47504101; GBAAT32777.1; glycosyl hydrolase, family 18 | 1,293 | 47.4 | + | + |
| gi47504599; GBAAT33275.1; cytochrome c oxidase, subunit I | 1,836 | 67.3 | + | − |
| Translation | ||||
| gi47500532; GBAAT29208.1; ribosomal protein L30 | 183 | 6.7 | − | + |
| gi47500548; GBAAT29224.1; ribosomal protein S9 | 393 | 14.4 | ++ | ++ |
| gi47504277; GBAAT32953.1; host factor-I protein | 225 | 8.3 | + | ++ |
| gi47504383; GBAAT33059.1; ribosomal protein S15 | 270 | 9.9 | − | + |
| gi47502550; GBAAT31226.1; ATP-dependent RNA helicase, DEAD/DEAH box family | 1,170 | 42.9 | + | − |
| Control | ||||
| Exosporium preparation | ++++ | ++++ | ||