Table II.
Proteins identified from B. anthracis 34F2 Sterne spore
The spot number indicates the corresponding protein spot in the 2D electrophoretic map shown in Fig. 4.
| Spot no. | Locus tag (GBAA) | Accession no. (gi) | Protein | No. of peptides matched | Sequence coverage | Theoretical molecular mass (kDa)/pI |
|---|---|---|---|---|---|---|
| % | ||||||
| Cell wall/surface | ||||||
| 1 | 1094 | 47526368 | Wall-associated protein, putative (RhsA) | 16 | 32 | 249.01/5.97 |
| 2 | 0887 | 47526173 | S-layer protein EA1 precursor (Eag) | 21 | 31 | 91.36/5.70 |
| 3 | 0252 | 47525509 | Alanine racemase (Dal-1/Alr) | 12 | 36 | 43.66/5.51 |
| Translation | ||||||
| 4 | 0107 | 47525363 | Translation elongation factor G (FusA) | 25 | 36 | 76.33/4.91 |
| 5 | 3950 | 47529240 | Translational initiation factor (InfB) | 23 | 33 | 75.75/5.03 |
| 6 | 0108 | 47525364 | Translation elongation factor Tu (Tuf) | 28 | 53 | 42.93/4.93 |
| 7 | 3964 | 47529254 | Translation elongation factor Ts (Tsf) | 22 | 45 | 32.30/5.25 |
| Protein folding assembly | ||||||
| 8 | 4539 | 47529836 | Molecular chaperone DnaK | 16 | 40 | 65.76/4.65 |
| 9 | 0267 | 47525527 | Chaperonin GroEL | 23 | 31 | 57.43/4.79 |
| 10 | 4540 | 47529837 | Hsp70 cofactor, GrpE protein | 11 | 61 | 21.52/4.56 |
| 11 | 0266 | 47525525 | Co-chaperonin GroES | 7 | 37 | 10.06/4.69 |
| Energy metabolism | ||||||
| 12 | 4843 | 47530138 | Pyruvate kinase (PykA-2) | 14 | 55 | 62.10/5.20 |
| 13 | 0309 | 47500720 | Δ1-Pyrroline-5-carboxylate dehydrogenase | 22 | 31 | 56.22/5.43 |
| 14 | 0008 | 47525264 | Inosine-5-monophosphate dehydrogenase (GuaB) | 17 | 47 | 52.37/6.30 |
| 15 | 4385 | 47529680 | Dihydrolipoamide dehydrogenase (BfmbC) | 33 | 51 | 50.77/5.53 |
| 16 | 5367 | 47530678 | Phosphoglycerate kinase (Pgk) | 21 | 61 | 42.29/5.14 |
| 17 | 5716 | 47531053 | Adenylosuccinate synthetase (PurA) | 18 | 60 | 47.42/5.57 |
| 18 | 4184 | 47529480 | Pyruvate dehydrogenase, E1α (PdhA) | 14 | 63 | 41.44/5.52 |
| 19 | 5369 | 47530679 | Glyceraldehyde-3-PO4 dehydrogenase (Gap-2) | 10 | 35 | 35.82/5.37 |
| 20 | 4183 | 47529479 | Pyruvate dehydrogenase, E1β (PdhB) | 12 | 32 | 35.22/4.75 |
| 21 | 5364 | 47530674 | Enolase (Eno) | 13 | 43 | 46.41/4.66 |
| Amino acid biosynthesis | ||||||
| 22 | 5387 | 47778397 | Thioredoxin reductase B (TrxB) | 9 | 47 | 34.66/5.16 |
| 23 | 0131 | 47525387 | Adenylate kinase (Adk) | 6 | 35 | 23.74/4.91 |
| Sporulation | ||||||
| 24 | 0803 | 47526092 | Coat protein JC (CotJC) | 8 | 51 | 21.65/5.15 |
| 25 | 0053 | 47525307 | Stage V sporulation protein T (SpoVT) | 21 | 38 | 19.69/5.09 |
| 26 | 1238 | 47526503 | Spore coat protein Z (CotZ-2) | 13 | 31 | 16.84/4.71 |
| 27 | 4898 | 47530192 | Small acid-soluble spore protein B (SspB) | 6 | 53 | 6.80/6.13 |
| Nucleotide metabolism | ||||||
| 28 | 3944 | 47529234 | Polyribonucleotide nucleotidyltransferase (Pnp) | 11 | 36 | 78.20/5.16 |
| 29 | 5583 | 47530904 | CTP synthase (CtrA) | 25 | 34 | 59.75/5.28 |
| 30 | 5549 | 47530869 | ATP synthase, F1 (AtpA) | 25 | 39 | 54.64/5.30 |
| Cell signaling | ||||||
| 31 | 4499 | 47529794 | Mn-superoxide dismutase (SodA-1) | 7 | 58 | 22.66/5.31 |
| Cellular process | ||||||
| 32 | 0344 | 47525611 | Alkyl hydroperoxide reductase, F (AhpF) | 24 | 35 | 54.81/4.93 |
| 33 | 2541 | 47502979 | ABC transporter, ATP-binding protein | 8 | 40 | 25.00/5.93 |
| 34 | 5222 | 47505676 | ABC transporter, ATP-binding protein | 15 | 42 | 37.26/6.18 |
| Hypothetical protein | ||||||
| 35 | 1267 | 47526534 | Hypothetical protein | 23 | 31 | 73.25/5.29 |
| 36 | 0029 | 47525285 | Hypothetical protein | 11 | 39 | 31.76/5.38 |
| 37 | 0020 | 47525275 | Hypothetical protein | 9 | 59 | 11.86/5.17 |
| 38 | 0145 | 47525401 | Hypothetical protein | 10 | 45 | 17.45/5.82 |