Table V.
Sera-reactive conserved Bacillus spore proteins
+, spots present in Western immunoblot against anti-B. anthracis 34F2 ΔgerH spore; •, spots present in Western immunoblot against anti-B. anthracis 34F2 exospore; ▴, spots present in Western immunoblot against anti-whole spore (irradiated ungerminated B. anthracis Ames). Ba, B. anthracis; Bt, B. thuringiensis; Bc, B. cereus; Bm, B. mycoides; Bp, B. pseudomycoides.
| Spot no.a | Locus (GBAA) | Protein | Theoretical molecular mass (kDa)/pI | Ba 34F2 | Bt 4AJ1 | Bc 1293 | Bc 6E1 | Bc 10987 | Bc 10876 | Bt 4Y1 | Bc 1550 | Bm 2048 | Bp 12442 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 3601 | Conserved repeat domain protein (Crd) | 245.09/4.17 | + • ▴ | + ▴ | + • | + • ▴ | • | + • ▴ | + ▴ | + | + ▴ | |
| 2 | 1094 | Wall-associated protein, putative (RhsA) | 249.01/5.97 | + • ▴ | |||||||||
| 3 | 3677 | Aconitate hydratase 1 (AcnA) | 99.01/4.8 | + • ▴ | + | + ▴ | + | + | ▴ | ||||
| 4 | 0052 | Transcription-repair coupling factor (Mfd) | 134.15/5.36 | + • ▴ | |||||||||
| 5 | 4157 | Pyruvate decarboxylase (Pyc) | 128.57/6.0 | + • ▴ | |||||||||
| 6 | 0887 | S-layer protein EA1 precursor (Eag) | 91.36/5.70 | + • ▴ | + • ▴ | + • ▴ | + ▴ | + • ▴ | + ▴ | ||||
| 7 | 3944 | Polyribonucleotide nucleotidyltransferase (Pnp) | 78.20/5.16 | + • ▴ | |||||||||
| 8 | 4843 | Pyruvate kinase (PykA-2) | 62.10/5.20 | + • ▴ | |||||||||
| 9 | 5583 | CTP synthase (CtrA) | 59.75/5.28 | + ▴ | |||||||||
| 10 | 0309 | Δ1-Pyrroline-5-carboxylate dehydrogenase | 56.22/5.43 | + ▴ | |||||||||
| 11 | 3609 | Aldehyde dehydrogenase (DhaS) | 53.74/5.4 | + ▴ | |||||||||
| 12 | 4184 | Pyruvate dehydrogenase, E1α (PdhA) | 41.44/5.52 | + ▴ | |||||||||
| 13 | 0252 | Alanine racemase (Dal-1/Alr) | 43.66/5.51 | + ▴ | |||||||||
| 14 | 5222 | ABC transporter, ATP-binding protein (MetN) | 37.26/6.18 | + ▴ | |||||||||
| 15 | 4539 | Molecular chaperone DnaK | 65.76/4.65 | + | + | + ▴ | + • ▴ | + ▴ | + • ▴ | + | ▴ | ||
| 16 | 0267 | Chaperonin GroEL | 57.43/4.79 | + | + ▴ | ||||||||
| 17 | 0108 | Translation elongation factor Tu (Tuf) | 42.93/4.93 | + | |||||||||
| 18 | 0107 | Translation elongation factor G (FusA) | 76.33/4.91 | + | + | + ▴ | + • ▴ | + • ▴ | + | ▴ |
a
The spot number indicates the corresponding protein spot in the 2D electrophoretic map in Fig. 5.