Table VI.
Amino acid sequence similarity of orthologs in B. cereus s.l.
Cutoff, at least 50% protein identity over 100 amino acids. Protein identity is represented in percent. The length of the amino acid sequence hit is presented in parentheses. The bold portions represent the correlation of sera-reactive conserved Bacillus spore proteins in Table V. aa, amino acids; Ba, B. anthracis; Bt, B. thuringiensis; Bc, B. cereus; Bm, B. mycoides; Bp, B. pseudomycoides.
| Accession no. | Locus tag | Protein | Bt4AJ1 | Bc1293 | Bc6E1 | Bc10987 | Bc10876 | Bt4Y1 | Bc1550 | Bm2048 | Bp12442 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| gi47528887 | GBAA3601 | Conserved repeat domain protein (Crd), 2357 aa | 72.36 (1616) | 71.87 (1616) | 71.19 (1616) | 69.71 (1616) | 71.19 (1616) | 90.65 (213) | 69.62 (1616) | 69.34 (1616) | — |
| 80.33 (2326) | 79.16 (2326) | 79.50 (2326) | 77.09 (2325) | 78.39 (2326) | 93.82 (468) | 77.80 (2326) | 77.80 (2326) | ||||
| 91.39 (1729) | |||||||||||
| gi47526368 | GBAA1094 | Wall-associated protein, putative (RhsA), 2223 aa | — | — | 76.81 (265) | 55.42 (162) | — | — | 57.14 (209) | — | — |
| 50.19 (237) | 85.45 (430) | ||||||||||
| 76.47 (318) | |||||||||||
| 82.66 (2210) | |||||||||||
| 86.82 (2107) | |||||||||||
| gi47528961 | GBAA3677 | Aconitate hydratase 1 (AcnA), 906 aa | 100.00 (906) | 99.67 (906) | 100.00 (906) | 99.56 (906) | 99.89 (906) | 99.67 (906) | 99.67 (906) | 97.68 (906) | 97.02 (906) |
| gi47525306 | GBAA0052 | Transcription-repair coupling factor (Mfd), 1175 aa | 99.91 (1175) | 98.95 (475) | 99.83 (578) | 99.74 (1175) | 97.96 (1175) | 95.64 (320) | 97.96 (1175) | 97.62 (1175) | 89.50 (475) |
| 98.04 (712) | 99.50 (602) | 97.10 (826) | 97.53 (687) | ||||||||
| gi47529455 | GBAA4157 | Pyruvate decarboxylase (Pyc), 1147 aa | 94.57 (220) | 98.98 (489) | 99.30 (1147) | 99.13 (1147) | 97.53 (1092) | 99.13 (1147) | 98.10 (209) | 97.96 (489) | 55.38 (128) |
| 99.57 (939) | 98.80 (663) | 98.29 (937) | 96.84 (663) | 94.39 (1087) | |||||||
| gi47526173 | GBAA0887 | S-layer protein EA1 precursor (Eag), 861 aa | 59.88 (171) | 53.26 (182) | 52.11 (210) | 58.72 (171) | 52.94 (135) | 59.88 (171) | 51.53 (195) | 60.47 (171) | — |
| 51.53 (195) | 59.30 (171) | 55.78 (198) | 53.16 (188) | 53.10 (144) | 52.11 (210) | 54.27 (198) | |||||
| 56.35 (195) | 60.13 (152) | 88.53 (861) | 56.12 (195) | 53.77 (206) | 57.14 (195) | ||||||
| 55.78 (198) | 56.12 (195) | 56.28 (198) | 55.78 (198) | ||||||||
| 99.88 (861) | 87.69 (267) | 88.79 (854) | |||||||||
| 83.73 (165) | |||||||||||
| 87.24 (383) | |||||||||||
| gi47529234 | GBAA3944 | Polyribonucleotide nucleotidyltransferase (Pnp), 711 aa | 99.71 (693) | 99.28 (693) | 100.00 (115) | 99.14 (693) | 99.42 (693) | 99.86 (693) | 99.42 (693) | 96.69 (693) | 95.39 (693) |
| 99.83 (578) | |||||||||||
| gi47530138 | GBAA4843 | Pyruvate kinase (PykA-2), 584 aa | 100.00 (584) | 99.63 (540) | 100.00 (584) | 99.66 (584) | 99.66 (584) | 99.55 (223) | 96.05 (584) | 94.76 (190) | 98.46 (584) |
| 98.85 (346) | 97.77 (403) | ||||||||||
| gi47530904 | GBAA5583 | CTP synthase (CtrA), 534 aa | 99.63 (534) | 99.44 (534) | 99.63 (534) | 99.25 (534) | 98.69 (534) | 99.02 (509) | 98.88 (534) | 98.50 (534) | 97.76 (534) |
| gi47500720 | GBAA0309 | Δ1-Pyrroline-5-carboxylate dehydrogenase, 514 aa | 100.00 (514) | 100.00 (514) | 100.00 (514) | 99.81 (514) | 99.42 (514) | 99.81 (514) | 99.49 (197) | 98.72 (233) | 96.31 (514) |
| 99.37 (317) | 95.73 (280) | ||||||||||
| gi47528895 | GBAA3609 | Aldehyde dehydrogenase (DhaS), 493 aa | 64.96 (487) | 65.16 (487) | 64.96 (487) | 65.16 (487) | 63.89 (492) | 65.16 (487) | 64.56 (157) | 64.55 (487) | 65.37 (487) |
| 100.00 (493) | 98.58 (493) | 100.00 (493) | 98.58 (493) | 98.58 (493) | 98.99 (493) | 65.73 (320) | 97.57 (493) | 91.76 (169) | |||
| 98.58 (493) | 95.08 (324) | ||||||||||
| gi47529480 | GBAA4184 | Pyruvate dehydrogenase, E1α (PdhA), 372 aa | 100.00 (370) | 100.00 (370) | 100.00 (370) | 100.00 (370) | 98.92 (370) | 100.00 (367) | 98.92 (370) | 99.69 (323) | 99.73 (370) |
| gi47525509 | GBAA0252 | Alanine racemase (Dal-1/Alr), 388 aa | 99.74 (388) | 99.23 (388) | 99.74 (388) | 99.49 (388) | 96.40 (388) | 99.23 (388) | 96.66 (388) | 95.89 (388) | 87.40 (388) |
| gi47505676 | GBAA5222 | ABC transporter, ATP-binding protein, methionine transport (MetN) 340 aa | 100.00 (340) | 99.71 (340) | 94.41 (340) | 99.41 (340) | 99.12 (340) | 100.00 (340) | 99.12 (340) | 98.43 (254) | 88.56 (340) |
| gi47529836 | GBAA4539 | Molecular chaperone DnaK, 610 aa | 100.00 (610) | 98.20 (610) | 98.79 (164) | 98.04 (610) | 99.67 (610) | 99.84 (610) | 100.00 (231) | 98.69 (610) | 95.91 (610) |
| 99.56 (451) | 99.73 (368) | ||||||||||
| gi47525527 | GBAA0267 | Chaperonin GroEL, 543 aa | 99.81 (530) | 99.81 (530) | 99.81 (530) | 97.24 (180) | 99.25 (530) | 99.25 (530) | 99.25 (530) | 97.93 (530) | 98.31 (530) |
| 100.00 (353) | |||||||||||
| gi47525364 | GBAA0108 | Translation elongation factor Tu (Tuf), 394 aa | 100.00 (394) | 99.75 (394) | 100.00 (394) | 99.75 (394) | 100.00 (394) | 100.00 (394) | 100.00 (146) | 97.39 (305) | 98.73 (393) |
| 100.00 (247) | |||||||||||
| gi47525363 | GBAA0107 | Translation elongation factor G (FusA), 691 aa | 100.00 (691) | 99.42 (691) | 100.00 (689) | 99.42 (691) | 99.42 (691) | 99.42 (691) | 99.42 (691) | 98.12 (691) | 98.70 (691) |