Table II.
List of 48 proteins identified by two or more peptides in the exosome fraction of the PC346C cell line
Annotations are derived from the Gene Ontology database.
| Protein name | Gene symbol | Protein accession no. | No. of unique peptides | Sequence coverage | Molecular function | Biological process | Location |
|---|---|---|---|---|---|---|---|
| % | |||||||
| Actin, γ 1 | ACTG1 | IPI00021440 | 15 | 40.30 | Structural constituent of cytoskeleton | Cell motility | Cytoplasm |
| ADAM metallopeptidase domain 10 | ADAM10 | IPI00013897 | 3 | 3.88 | Protein homodimerization activity | Protein amino acid phosphorylation | Cell membrane |
| ADAM metallopeptidase domain 15 | ADAM15 | IPI00013302 | 4 | 6.39 | Proteolysis and peptidolysis | Cell adhesion | Cell membrane |
| Annexin A2 | ANXA2 | IPI00418169 | 7 | 24.90 | Phospholipase inhibitor activity | Skeletal development | Secreted protein |
| Annexin A6 | ANXA6 | IPI00002459 | 3 | 7.20 | Calcium ion binding | ||
| ATPase, Na+/K+-transporting, α 1 polypeptide | ATP1A1 | IPI00006482 | 5 | 5.96 | Sodium:potassium-exchanging ATPase activity | Sodium ion transport | Cell membrane |
| Brain-abundant, membrane-attached signal protein 1 | BASP1 | IPI00299024 | 4 | 40.10 | Cell membrane | ||
| Chromosome 1 open reading frame 58 | C1orf58 | IPI00065500 | 2 | 10.70 | |||
| Capping protein (actin filament) muscle Z-line, α 1 | CAPZA1 | IPI00005969 | 2 | 7.69 | Actin binding | Cell motility | |
| CD151 molecule (Tetraspanin 24) | CD151 | IPI00298851 | 2 | 5.93 | Protein binding | Cell adhesion | Cell membrane |
| CD2-associated protein | CD2AP | IPI00412771 | 6 | 11.60 | Structural constituent of cytoskeleton | Cell migration | Cytoplasm |
| CD9 molecule | CD9 | IPI00215997 | 3 | 15.40 | Protein binding | Cell motility | Cell membrane |
| Chromatin-modifying protein 4B | CHMP4B | IPI00025974 | 2 | 8.93 | Cytoplasm | ||
| Clathrin, heavy chain 1 | CLTC | IPI00024067 | 10 | 6.81 | Signal transducer activity | Receptor-mediated endocytosis | Cell membrane |
| Eukaryotic translation elongation factor 1 α 1 | EEF1A1 | IPI00396485 | 14 | 37.20 | Translation elongation factor activity | Translation elongation | Cytoplasm |
| EH domain-containing protein 1 | EHD1 | IPI00017184 | 2 | 5.06 | ATP binding | Cell membrane | |
| F11 receptor | F11R | IPI00001754 | 5 | 16.40 | Cell motility | Cell membrane | |
| Family with sequence similarity 125, member A | FAM125A | IPI00744702 | 3 | 16.10 | Cytoplasm | ||
| Formin-binding protein 1-like | FNBP1L | IPI00015580 | 2 | 3.47 | Cytoplasm | ||
| Folate hydrolase (prostate-specific membrane antigen) 1 | FOLH1 | IPI00028514 | 7 | 12.30 | Dipeptidase activity | Proteolysis | Cell membrane |
| Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | IPI00219018 | 3 | 14.00 | Glyceraldehyde-3-phosphate dehydrogenase activity | Glycolysis | Cytoplasm |
| Histone cluster 1, H1c | HIST1H1C | IPI00217465 | 2 | 10.30 | DNA binding | Nucleosome assembly | Nucleus |
| Histone cluster 1, H2ab | HIST1H2AB | IPI00026272 | 2 | 21.50 | DNA binding | Nucleosome assembly | Nucleus |
| Heat shock protein 90 kDa α (cytosolic), class B member 1 | HSP90AB1 | IPI00334775 | 4 | 6.49 | Unfolded protein binding | Response to unfolded protein | Cytoplasm |
| Heat shock 70-kDa protein 1B | HSPA1B | IPI00807640 | 6 | 16.50 | Unfolded protein binding | Antiapoptosis | Cytoplasm |
| Heat shock 70-kDa protein 8 | HSPA8 | IPI00003865 | 13 | 25.50 | ATPase activity | Protein folding | Cytoplasm |
| Immunoglobulin superfamily, member 8 | IGSF8 | IPI00056478 | 6 | 12.60 | Protein binding | Cell motility | Cell membrane |
| Integrin β-1 | ITGB1 | IPI00217563 | 3 | 3.89 | Protein heterodimerization activity | Cell migration | Cell membrane |
| Lactate dehydrogenase B | LDHB | IPI00219217 | 2 | 7.78 | l-Lactate dehydrogenase activity | Anaerobic glycolysis | Cytoplasm |
| Milk fat globule-EGF factor 8 protein | MFGE8 | IPI00002236 | 2 | 4.91 | Cell adhesion | Cell membrane | |
| Poly(A)-binding protein, cytoplasmic 1 | PABPC1 | IPI00008524 | 16 | 25.90 | Translation activator activity | mRNA stabilization | Cytoplasm |
| Poly(A)-binding protein, cytoplasmic 4 | PABPC4 | IPI00555747 | 5 | 18.40 | Protein/RNA binding | RNA processing | Cytoplasm |
| Protein kinase C and casein kinase substrate in neurons 2 | PACSIN2 | IPI00027009 | 4 | 8.85 | Transporter activity | Intracellular protein transport | Cytoplasm |
| Poly(rC)-binding protein 2 | PCBP2 | IPI00012066 | 2 | 7.46 | Protein/RNA binding | mRNA metabolic process | Cytoplasm |
| Programmed cell death 6-interacting protein | PDCD6IP | IPI00246058 | 21 | 22.40 | Signal transducer activity | Apoptosis | Cytoplasm |
| Prostaglandin F2 receptor negative regulator | PTGFRN | IPI00022048 | 7 | 9.33 | Protein binding | Negative regulation of protein biosynthetic process | Cell membrane |
| % | |||||||
| Ribosomal protein S27a | RPS27A | IPI00179330 | 5 | 31.40 | Structural constituent of ribosome | Translation | Cytoplasm |
| Syndecan-binding protein (syntenin) | SDCBP | IPI00299086 | 3 | 15.10 | Protein heterodimerization activity | Cell migration | Cell membrane |
| Serine incorporator 5 | SERINC5 | IPI00328883 | 2 | 4.97 | Cell membrane | ||
| SH3-domain GRB2-like 1 | SH3GL1 | IPI00019169 | 8 | 23.60 | Protein binding | Signal transduction | Cell membrane |
| Solute carrier family 3, member 2 | SLC3A2 | IPI00027493 | 2 | 5.48 | Catalytic activity | Amino acid transport | Cell membrane |
| Sphingomyelin phosphodiesterase, acid-like 3B | SMPDL3B | IPI00550115 | 2 | 4.73 | Hydrolase activity | Carbohydrate metabolism | Secreted protein |
| Sorting nexin-9 | SNX9 | IPI00001883 | 2 | 5.38 | Protein binding | Protein localization | |
| Tumor-associated calcium signal transducer 1 | TACSTD1 | IPI00296215 | 5 | 21.30 | Cell membrane | ||
| Tumor susceptibility gene 101 protein | TSG101 | IPI00018434 | 7 | 21.30 | Transcription corepressor activity | Regulation of cell growth | Cytoplasm |
| Tubulin, β | TUBB | IPI00011654 | 3 | 7.43 | Structural constituent of cytoskeleton | Spindle assembly | |
| Vacuolar protein sorting 37 homolog B | VPS37B | IPI00002926 | 3 | 10.20 | |||
| Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, ζ polypeptide | YWHAZ | IPI00021263 | 5 | 22.90 | Transcription factor binding | Signal transduction | Cytoplasm |