Table III.

Summary of sample fractions applied per MS measurement

The number of fractions generated by protein prefractionation (column 2), type of chromatography used in peptide separation (column 3), HPLC elution steps (column 4), total number of in-line HPLC elution steps per measurement (column 5), and number of peptides from the proteins identified by each MS step (column 6) are listed by method.

Method	Pre-MS fraction	Nano-HPLC dimension	Peptide elution step	Total MS steps	No. of peptides/step
2DC	1	2 (SCX-RP)	17	17	171
GRPC	43–50	1 (RP)	1	43–50	106
GRPT	70–80	1 (RP)	1	70–80	253
G2DC	7	2 (SCX-RP)	17	119	308