Fig. 4.

Subcellular localization of the tagged proteins and the composition of the protein complexes associated with them in yeast cells. The co-purified proteins were separated by SDS-PAGE and stained with colloidal Coomassie. Proteins were identified by a standard in-gel digestion/mass spectrometry procedure. A, subcellular localizations of Mrp8-GFP-3×FLAG-His₆ and Lsp1-mCherry-3×MYC-His₈ and the major components of the protein complexes purified with the tagged Mrp8. B, subcellular localization of Ygr130c-GFP-3×FLAG-His₆ and the major components of the complexes co-purified with the tagged protein. C, subcellular localization of Ymr031c-GFP-3×FLAG-His₆ and the composition of the major components of complexes co-purified with the tagged protein. D, subcellular localization of Ymr086-GFP-3×FLAG-His₆ and the major components of the complexes purified with the tagged protein. The full list of the identified proteins, unique peptides found, and protein coverage can be found in the supplemental material.