Table III.
LC-MS analysis of SDS-PAGE gel bands from whole worm extracts and ESPs of S. japonicum
aa, amino acids; Seq, sequence; WCL-abd, abundant in WCL extract of adult worms; ES-abd, abundant in ES products; WCL-ag, antigenic in WCL extract of adult worms; ES-ag, antigenic in ES products; SMS, summed Mowse score.
| Gel band no. | Category | Gi (NCBI) no. | Annotation | Protein length (aa) | Seq coverage | SMS | SC |
|---|---|---|---|---|---|---|---|
| aa | % | ||||||
| 1 | WCL-abd | 1703114 | Actin-2 | 376 | 33.5 | 1381.2 | 27 |
| 1 | WCL-abd | 30995481 | β-Tubulin | 443 | 11.7 | 239.12 | 5 |
| 1 | WCL-abd | 349802 | Enolase | 434 | 2.3 | 225.76 | 4 |
| 1 | WCL-abd | 123599 | HSP70 | 198 | 9.6 | 160.23 | 3 |
| 1 | WCL-abd | 160996 | GAPDH | 338 | 5.9 | 181.04 | 3 |
| 1 | WCL-abd | 161072 | α-Tubulin | 451 | 3.3 | 179.08 | 3 |
| 1 | WCL-abd | 38683290 | Actin-binding/filamin-like protein | 984 | 1.5 | 177.33 | 3 |
| 1 | WCL-abd | 84402 | Glutathione transferase | 219 | 10.5 | 168.27 | 3 |
| 1 | WCL-abd | 95113705 | Fructose-1,6-bisphosphate aldolase | 363 | 6.6 | 193.26 | 3 |
| 2 | WCL-abd | 2829289 | HSP70 | 648 | 15.9 | 708.32 | 11 |
| 2 | WCL-abd | 399939 | HSP70 homolog | 637 | 7.1 | 197.05 | 3 |
| 3 | WCL-abd | 3941320 | Myosin | 802 | 20.0 | 2065.5 | 33 |
| 3 | WCL-abd | 3041711 | Paramyosin (antigen Sj97) | 866 | 20.7 | 328.36 | 6 |
| 4 | WCL-ag | 3041711 | Paramyosin (antigen Sj97) | 866 | 20.7 | 2861.1 | 47 |
| 5 | WCL-ag | 146741272 | Paramyosin | 866 | 10.6 | 474.02 | 8 |
| 5 | WCL-ag | 3041711 | Paramyosin (antigen Sj97) | 866 | 20.7 | 332.14 | 5 |
| 5 | WCL-ag | 56753909 | Oncosphere protein Tso22b | 328 | 12.2 | 322.81 | 5 |
| 5 | WCL-ag | 56759038 | HSP90α2 | 719 | 4.2 | 213.19 | 4 |
| 5 | WCL-ag | 56756044 | His-tagged cytosolic leucine aminopeptidase | 398 | 3.8 | 133.36 | 2 |
| 6 | WCL-abd | 76154815 | Myosin heavy chain | 976 | 22.6 | 710.14 | 13 |
| 6 | WCL-abd | 56758294 | 14-3-3 protein | 254 | 4.7 | 117.71 | 2 |
| 7 | ES-ag | 76154815 | Myosin heavy chain | 976 | 22.6 | 2194.7 | 37 |
| 8 | ES-abd | 76155249 | Heat shock protein 70 | 137 | 21.2 | 201.03 | 4 |
| 8 | ES-abd | 76162587 | Similar to major vault protein | 111 | 12.6 | 308.21 | 4 |
| 8 | ES-abd | 56756008 | Ornithine aminotransferase | 438 | 8.4 | 160.93 | 3 |
| 8 | ES-abd | 56758570 | Mitochondrial malate dehydrogenase | 341 | 7.9 | 107.26 | 2 |
| 8 | ES-abd | 60688010 | Heat shock protein 70 | 82 | 17.1 | 137.26 | 2 |
| 9 | ES-abd | 56754704 | Actin-2 | 376 | 9.3 | 773.07 | 16 |
| 9 | ES-abd | 76154123 | Lamin b2 | 337 | 8.3 | 213.52 | 4 |
| 9 | ES-abd | 76152538 | Nuclear lamin C protein | 266 | 10.2 | 142.92 | 3 |
| 9 | ES-abd | 56757081 | Similar to major vault protein | 157 | 9.6 | 95.88 | 2 |
| 10 | ES-abd | 56758584 | ATP synthase subunit β, mitochondrial precursor | 514 | 11.9 | 296.46 | 6 |
| 10 | ES-abd | 56759082 | Mitochondrial H+-ATPase a subunit | 209 | 18.2 | 255.38 | 5 |
| 10 | ES-abd | 1174754 | Tropomyosin-1 | 284 | 12.0 | 115.56 | 2 |
| 11 | WCL-ag | 1174754 | Tropomyosin-1 | 284 | 12.0 | 2882.6 | 45 |
| 11 | WCL-ag | 1174756 | Tropomyosin-2 (TMII) | 284 | 12.7 | 229.88 | 4 |
| 11 | WCL-ag | 189502922 | 14-3-3 protein homolog 1 | 254 | 9.1 | 151.54 | 2 |