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. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: Oncogene. 2008 Sep 22;27(58):7274–7284. doi: 10.1038/onc.2008.333

Figure 3. Co-Immunostaining indicates an inverse correlation between EZH2 and E-cadherin in cell lines and tumors.

Figure 3

(a) Immunostaining of the breast cell line H16N2 infected with EZH2 and control adenovirus (i, ii, iii and iv). Green staining represents EZH2 protein, red staining represents E-cadherin, and blue represents nuclear staining with DAPI. Lower right panel (iv) shows a higher magnification image. (b) Association between EZH2 and E-cadherin protein levels in human breast tumors by immunofluorescence. Upper left panel (i) shows invasive carcinoma of the breast with high EZH2 protein expression in the nuclei (green) and low E-cadherin expression (red), evidenced by a decrease in the membrane staining. A normal lobule is present in the lower part of the figure. Top right panel (ii) shows the higher magnification of the normal lobule shown with crisp membrane staining for E-cadherin. EZH2 staining is absent in this region. The lower left panel (iii) shows invasive carcinoma with foci of high EZH2 expression. The lower right panel (iv) shows higher magnification of a focus of invasive carcinoma with high EZH2 expression and nearly absent E-cadherin staining.