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. 2009 May 5;5:2. doi: 10.1186/1745-7580-5-2

Figure 8.

Figure 8

Direct and competitive LOCI-based peptide-MHC-II binding assay. Titrations on a promiscuous malaria derived peptide YILLKKILSSRFNQM DR4: KD = 133 nM R2 = 0.98. DP401: KD = 0.8 nM R2 = 0.99. I-Ed: KD = 14 nM R2 = 0.98. DR2a: inhibition assay, reference peptide 4 nM Biotin-PEG-HA306–318 IC50 = 0.6 nM R2 = 0.98.