Table 1.
Affinity measurements using LOCI assay.
|
DR1GrpEa Kompb |
DR2a Kompb |
DR2a pH6 | DR3 | DR4 | DRB1*0813 | DRB3*0301 | DQA1*0501 DQB1*0201 | DP401 | I-ED | Reference | ||
| Designation | Sequence | IC50 (nM) |
IC50 (nM) |
nM | nM | nM | nM | nM | nM | nM | nM | |
| MBP | FLYGALLLA | 3921 | NB | NB | NB | NB | NB | N.D | N.D | 34 | NB | [26] |
| TERT572Y | YLFFYRKSV | 4287 | NB | 2648 | NB | 1593 | 95 | N.D | N.D | NB | 322 | |
| Flu PR8 derived | FMYSDFHFI | 642 | 1018 | 637 | NB | 1130 | NB | N.D | N.D | 1 | NB | [27] |
| Pox.o42.con A2 | FLIDLAFLI | NB | 4276 | NB | NB | NB | NB | NB | NB | 179 | NB | |
| Pox.o42.con.im. A2 | RMIAISAKV | 1464 | 1985 | 1647 | 516 | NB | 267 | 1259 | NB | NB | NB | |
| Brucella | WMARRHAER | NB | NB | NB | NB | NB | 224 | NB | NB | NB | 6 | |
| Salmonella | MMAWRMMRY | 7 | 25 | 18 | 1709 | 68 | 28 | 1426 | 4318 | NB | 837 | |
| Flu HA1194-202 | IVFMWAIHH | 42 | 529 | 167 | NB | NB | 276 | NB | NB | NB | NB | |
| Hantaan | FMSRKLHRY | NB | 2552 | NB | 279 | NB | 21 | NB | NB | 3 | ||
| Oxytocinase271 | YEKKYFAATQFEPLAARL | 5 | 29 | 6 | 38 | 6 | 38 | 33 | 1090 | 1 | NB | [79] (DP401) |
| Thyroid peroxidase | YIDVWLGGLAENFLPY | 605 | 691 | 2138 | NB | NB | NB | 3184 | 564 | 602 | NB | [6] |
| No nat. source | YPFIEQEGPEFFDQE | 1279 | NB | NB | 289 | 3972 | NB | 3228 | 648 | NB | NB | [6] |
| Hepatitis B | YLCQVFADATPTGWGL | 1850 | NB | 3703 | 69 | 18 | 2988 | 2058 | 1335 | 13 | NB | [6] (DR3, DR4) |
| Plasmodium Falciparum | YILLKKILSSRFNQM | 7 | 2 | 4 | 32 | 94 | 4 | 1 | NB | 1 | 20 | [28](DR1, DR2a, DR4) |
| No natural source | YILFLVKMNALRRLPV | 10 | 20 | 5 | 39 | ? | 78 | 3 | NB | 45 | 582 | [28] (DR1, DR2a, DR4) |
| Hepatitis B | VGNFTGLYSSTVPVF | 3 | 31 | 102 | 1414 | 632 | 2892 | 9 | 635 | NB | NB | |
| Influenza | PLKAEIAQRLEDV | 50 | 41 | 65 | 134 | 2678 | NB | 11 | NB | 2091 | NB | [29] (DR1, DR2a) |
| HIV | PIVQNIQGQMVHQ | 71 | 852 | 447 | 471 | NB | NB | 23 | NB | NB | NB | [30](DR1) |
| HepatitisB | PDRVHFASPLHVAWR | 5 | 26 | 8 | 6 | 881 | 39 | 3 | 2807 | 124 | NB | |
| Clostridium tetanus | QYIKANSKFIGITE | 18 | 11 | 22 | 30 | 432 | 29 | 1 | NB | 56 | 1501 | [29] (DR1, DR2a) |
| No nat. source | KILEPFRKYTAFTIP | 10 | 1 | 16 | 18 | 1600 | 11 | 1015 | 1542 | 30 | 2198 | [34] (DR2a) |
| Hepatitis B | AFSYMDDVVLGAKSV | 215 | 3382 | 1721 | 87 | 26 | 2753 | 764 | 2039 | NB | NB | |
| Mycobact leprea | GVTYEIDLTNKN | NB | NB | NB | 4346 | 2548 | NB | NB | NB | NB | NB | [6] (DR4) |
| Murine β2m | IQMLKNGKKIPKVEMS | 1232 | 3 | 6 | 21 | NB | 73 | 70 | NB | NB | 117 | [31] (I-Ed) |
| Lambda repressor | YLEDARRLKAIYEKKK | 2999 | 34 | 87 | 1098 | 3120 | 136 | NB | NB | NB | 184 | [31] (I-Ed) |
| 6xH Lambda 2(315) | HHHHHHIEGRFAALWFRNHFVFGGGTK | 4 | 20 | 5 | 882 | 11 | 8 | 1198 | NB | 2 | 26 | [32] |
| 6xH Influenza A (PR8) HA 109–119 | YHHHHHHIEGRSFERFEIFPKE | 325 | 23 | 9 | 1429 | 1869 | 181 | 1528 | 3913 | 1 | 984 | [33] |
| CLIP 6xH | LPKPPKPVSKMRMATPLLMQALPMYHHHHHH | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 224 | 1 | 23 | |
| CLIP | YLPKPPKPVSKMRMATPLLMQALPM | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 381 | 4 | 72 | |
| HA306-318 | YKYVKQNTLKLAT | 7 | 2 | 10 | 13 | 8 | 11 | 4 | NB | NB | 25 | Most DR |
Values represent an average of 2–4 measurements. Affinity measurements higher than 5000 nM were categorized as non-binders (NB). N.D: Not determined. a: The DR1 allele used for these measurements was produced as a construct comprising amino acids 1–181 and 1–190 of α and β chain respectively fused C terminally to the dimerizing nucleotide exchange factor GrpE (see additional file 1), this construct was not biotinylated and used in the LOCI competition assay. b: Determined using competitive LOCI assay. The reference column also states the MHC alleles known to bind the peptide.