Table 1.
Cytopathic effects (CPE) produced by parental and progeny virus strains in mammalian cell lines.
| Cytopathic Effects (CPE)a | ||||||||
| Cell line | Species/tissue | CPXV-NOH1 | MVA-HANP | Rec1 | Rec 2 | Rec 3 | Rec 3a | Rec 3b |
| IEC-6 | Rat/small intestine; normal | +++ | ++ | +++ | ++++ | ++ | ++++ | ++++ |
| BHK-21 | Hamster syrian/kidney; normal | ++ | +++ | ++++ | ++++ | ++ | ++++ | ++++ |
| Caco-2 | Human/colon; colorectal adenocarcinoma | +++ | - | + | ++ | +++ | +++ | ++++ |
| H411E | Rat/liver; hepatoma | ++ | + | ++ | +++ | + | +++ | +++ |
| FHs74int | Human/small intestine; normal | +++ | - | ++ | + | ++ | +++ | ++++ |
| Hutu-80 | Human/duodenum; adenocarcinoma | ++ | - | ++++ | ++++ | ++ | ++++ | ++++ |
| Vero | African Green Monkey/kidney; normal | +++ | + | ++++ | ++ | + | ++++ | ++++ |
| RK-13 | Rabbit/kidney; normal | ++ | + | ++++ | +++ | ++++ | +++ | ++++ |
| CHO-K1 | Hamster Chinese/ovary | ++ | - | +++ | ++++ | +++ | +++ | +++ |
| A549 | Human/lung; carcinoma | + | - | ++ | ++ | + | ++ | ++ |
| PK15 | Pig/kidney; normal | ++ | + | ++ | ++ | + | ++ | +++ |
| NMULI | Mouse/liver; normal | ++ | - | +++ | +++ | + | ++++ | ++++ |
| HEK-293 (CRL-1573) | Human/kidney; transformed with adenovirus 5 DNA | +++ | - | +++ | ++ | + | ++ | ++ |
a Virus infection was at a m.o.i of 0.05 pfu per cell. CPE was categorized on the following criteria: no difference from uninfected cells (-); low, < 25% CPE (+); moderate, 25 to 50%CPE (++); high, > 50 to 75% CPE (+++); very high, > 75 to 100% CPE or high level of cell detachment (++++) [25].