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. 2009 Jun 3;106(24):9842–9847. doi: 10.1073/pnas.0904464106

Fig. 1.

Fig. 1.

Five of 7 mouse Fpr genes are expressed in the VNO. qPCR was conducted using primers specific for each mouse Fpr gene, a mouse V1r gene (V1rd6), a mouse Taar gene (Taar7b), or the mouse β-Actin gene and, as templates, cDNAs prepared from DNase-treated RNAs from different mouse tissues: A, heart; B, spleen; C, intestine; D, liver; E, brain; F, VNO (red); G; olfactory epithelium (blue); H, circumvallate taste papillae; I, olfactory bulb; and J, testis). Results of triplicate experiments are shown (±SD). No PCR products were seen in control experiments lacking reverse transcriptase. cDNAs for five of the seven mouse Fpr genes (Fpr-rs1, Fpr-rs3, Fpr-rs4, Fpr-rs6, and Fpr-rs7) were selectively amplified from VNO cDNA (red bars), similar to V1rd6 cDNAs. Scales on the y axis differ as follows: X = 60,000 copies, Y = 100,000 copies, and Z = 8,000,000 copies. Each column represents signal from cDNA prepared from 1 μg total RNA, based on reactions using 10 ng RNA.