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. 2009 Jun 12;5(6):e1000469. doi: 10.1371/journal.ppat.1000469

Figure 3. EBNA1(1-450)-E2DBD does not squelch transactivation by EBNA1.

Figure 3

C33a cells were co-transfected with the FR-TKp-Luciferase reporter plasmid, and control vector pcDNA3 (dark grey bars), an expression plasmid for EBNA1(1-450)-E2DBD (light grey bars), or an expression plasmid for DBD (open bars, in the amounts indicated below each bar. Cells were harvested at 48 hours post-transfection, normalized by flow cytometry for the number of live-transfected cells, and analyzed for luciferase activity, which is expressed as percent luciferase activity relative to the activity obtained by co-transfection with the same amount pcDNA3.