Table 9. List of primers used in this study.
| Primers names | Primers sequences | Note | Source |
| CNACT1 | 5′ AATCTCGCCCAACATGT 3′ | Amplify ACT1 | This study |
| CNACT1R | 5′ TTAGAAACACTTTCGGTGGACG3′ | Amplify ACT1 | This study |
| CNACT1F2 | 5′ CCAAGCAGAACCGAGAGAAG 3′ | Internal primers of ACT1 | This study |
| URA5 | 5′ ATGTCCTCCCAAGCCCTCGACTCCG 3′ | Amplify URA5 | [8] |
| SJ101 | 5′ TTAAGACCTCTGAACACCGTACTC 3′ | Amplify URA5 | [8] |
| PLBCNAF | 5′ TAAAGTGCTTGGTGGGAACC 3′ | Amplify PLB1 from VN | This study |
| PLBCNAR | 5′ TCTCGCGAGGATTACAGGAT 3′ | Amplify PLB1 from VN | This study |
| PLBCG2F | 5′ TCCCCTTCAACACAGCTCTT 3′ | Amplify PLB1 from VG | This study |
| PLBCG2R2 | 5′ CACCTATCTTCGCTGCATCA 3′ | Amplify PLB1 from VG | This study |
| PLBCNIF1 | 5′ GGTTACCGTGCAATGCTGT 3′ | Internal primers of PLB1 | This study |
| PLBCNIF2 | 5′ GGTGCTTTCACCCCTATTGA 3′ | Internal primers of PLB1 | This study |
| PLBCNIR1 | 5′ CGGGAAATATCAGCTTGGTC 3′ | Internal primers of PLB1 | This study |
| IDEF | 5′ CCAAGGCGGACAAGGCTGCGG 3′ | Amplify IDE | [19] |
| IDER | 5′ GTAGAGGTGATCCATGTCGGG 3′ | Amplify IDE | [19] |
| ACT1CAF1 | 5′ GGTGTCATGGTCGGTATGG 3′ | Amplify ACT1 from CA | This study |
| ACT1CAR1 | 5′ GTACTTTCGCTCGGGAGGAG 3′ | Amplify ACT1 from CA | This study |
| ACT1CAR2 | 5′ AGCTTCTCCTTGATGTCTC 3′ | Amplify ACT1 from CA | This study |
| URA5DF1 | 5′ CCWTACTTCTTCAAYGCYGG 3′ | Amplify URA5 from FD | This study |
| MFLL | 5′ CTTCACTGCCATCTTCACCA 3′ | Mating type α determination of VN | [69] |
| MFLR | 5′ GACACAAAGGGTCATGCCA 3′ | Mating type α determination of VN | [69] |
| MFAL | 5′ CGCCTTCACTGCTACCTTCT 3′ | Mating type a determination of VN | [69] |
| MFAR | 5′ AACGCAAGAGTAAGTCGGGC 3′ | Mating type a determination of VN | [69] |
| MFαU | 5′ TTCACTGCCATCTTCACCACC 3′ | Mating type α determination of VG | [47] |
| MFαL | 5′ TCTAGGCGATGACACAAAGGG 3′ | Mating type α determination of VG | [47] |
| MFa2U | 5′ ACACCGCCTGTTACAATGGAC 3′ | Mating type a determination of VG | [38] |
| MFa2L | 5′ CAGCGTTTGAAGATGGACTTT 3′ | Mating type a determination of VG | [38] |
| SXI1αF | 5′ TACATCACCGGTCATATCTGC 3′ | Mating type α determination of VGIV | [68] |
| SXI1αR | 5′ CTGGAGAAGCGCCTCACTGGA 3′ | Mating type α determination of VGIV | [68] |
| SXI2aF | 5′ TGATCGCACGAGCCAAATCCC 3′ | Mating type a determination of VGIV | [68] |
| SXI2aR | 5′ GGCTTCCTGACAACACTTCTA 3′ | Mating type a determination of VGIV | [68] |
| GPD1F | 5′ CCACCGAACCCTTCTAGGATA 3′ | Amplify GPD1 | [70] |
| GPD1R | 5′ CTTCTTGGCACCTCCCTTGAG 3′ | Amplify GPD1 | [70] |
| IGS1F | 5′ ATCCTTTGCAGACGACTTGA 3′ | Amplify IGS1 | [14] |
| IGS1R | 5′ GTGATCAGTGCATTGCATGA 3′ | Amplify IGS1 | [14] |
Notes: VN = molecular type VNI, VNII and VNIV; VG = molecular type VGI, VGII, VGIII and IV, FD = Filobasidiella depauperata, CA = Cryptococcus albidus.