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. 2009 Jun 5;4(6):e5811. doi: 10.1371/journal.pone.0005811

Figure 7. Effects of (±) SKF83959 on the activation and steady-state inactivation of the delayed rectifier K+ current in rat hippocampal neurons.

Figure 7

(A) Activation curves of the delayed rectifier K+ current (I K) prior to and during superfusion with 100 µM (±) SKF83959 (n = 8). (B) Steady-state inactivation curves of I K prior to and during superfusion with 100 µM (±) SKF83959 (n = 8). (C) Time courses of recovery of I K from inactivation prior to and during superfusion with 100 µM (±) SKF83959 (n = 6). For studying the activation, neurons were held at −50 mV, currents were elicited with a series of 400-ms step from −80 mV to +80 mV in 10 mV increments following a 600-ms hyperpolarizing prepulse to −110 mV and a 50-ms interval at −50 mV, delivered every 10 s. For studying the steady-state inactivation, neurons were held at 0 mV, currents were elicited with a series of 600-ms prepulses at different hyperpolarizing potentials followed by a 50-ms interval at −50 mV and a 400-ms step to +40 mV, then back to 0 mV, delivered every 10 s. For studying the time course of recovery from inactivation, neurons were held at 0 mV, currents were elicited on return from hyperpolarizing prepulses of varying durations at −110 mV to +40 mV, delivered every 10 s.