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. 2009 Jun 11;4(6):e5873. doi: 10.1371/journal.pone.0005873

Figure 2. Intracellular iron concentration and MRI analyses of THP-1 and U251 phantom preparations.

Figure 2

Iron concentration in U251 (A) and THP-1 (B) cells that were labeled with 100 µg/ml Fe and 3 µg/ml Pro (red), 50 µg/ml Fe and 1.5 µg/ml Pro (blue), and 25 µg/ml Fe and 0.75 µg/ml Pro (green). Cells were incubated for 2, 4, 24 and 48 h at 37°C, 5% CO2. Non-labeled cells (black). Data expressed as means±SD. *p<0.05 for cells labeled with 100 µg/ml Fe and 3 µg/ml Pro for 4 h vs. cells labeled with 0–50 µg/ml Fe and 0–1.5 µg/ml Pro. R2* values of U251 (C) and THP-1 (D) cells labeled with 100 µg/ml Fe and 3 µg/ml Pro (star), 50 µg/ml Fe and 1.5 µg/ml Pro (square), and 25 µg/ml Fe and 0.75 µg/ml Pro (circle). Data expressed as means±SD. *p<0.05 for unlabeled vs. labeled cells. #p<0.05 THP-1 labeled with 50 µg/ml Fe and 1.5 µg/ml Pro for 4 h vs. THP-1 labeled with 50 µg/ml Fe and 1.5 µg/ml Pro for 24 h.