Skip to main content
. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Int J Oncol. 2009 Mar;34(3):743–755. doi: 10.3892/ijo_00000200

Figure 2.

Figure 2

Effect of thee time-spaced courses of 20 µM CDDP for 1 h on number, cell cycle kinetics, cell viability, and clonogenic survival of adherent OV2008 cells. Cells were seeded and allowed to attach to the plate for 48 h. Plates were then treated with vehicle (0.9 % NaCl) or 20 µM CDDP for 1 h (Day 0). CDDP was then removed and fresh medium was replaced. The treatment with CDDP was repeated on days 12 and 24. Every second day the floating cells were removed and fresh medium was added. Every 4 days, one aliquot of cells was exposed to exclusion fluorochomes to determine number of cells (a) and their percent viability (c) using a microcapillary cytometer. (b) A second cell aliquot was subjected to permeabilization, labeling with propidium iodide, and the cell cycle distribution was assessed. Represented are the kinetic distribution of cells with sub-G1 DNA content and cells with DNA content pertinent to S phase. (d) Five hundred viable cells were placed in 6-well plates and cultured for 7 days to assess for colony formation (clonogenic survival). Arrows, intermittent 1-h CDDP treatment. Values represent the means of a representative experiment performed in triplicates ± SEM. This experiment was repeated five times with similar outcomes. *, P < 0.05 relative to values on day 16; #, P < 0.05 relative to values on day 4; †, P < 0.05 relative to values on day 28; §, P < 0.05 relative to values on days 16 and 20.