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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Int J Oncol. 2009 Mar;34(3):743–755. doi: 10.3892/ijo_00000200

Figure 3.

Figure 3

Effect of chronic cytostatic concentrations of MF on the fate of OV2008 cells after exposure to one round of CDDP. Cells were seeded and allowed to attach to the plate surface for 48 h and thereafter treated with 20 µM CDDP for 1 h. The drug-containing medium was then removed and fresh medium with 0, 5, 10, or 20 µM MF was replaced every 2 days. Every 4 days number of adherent cells was measured by microcapillary cytometry (a), whereas the clonogenic capacity of 250 viable cells was studied in a clonogenic survival assay (b). Values represent the means of a representative experiment performed in triplicates ± SEM. This experiment was repeated 3 times with similar outcomes. *, P < 0.05 relative to MF-free control values on day 4; †, P < 0.05 relative to time-matched MF-free controls.