Table 1.

Cells that repopulated after 3 rounds of CDDP grow at a similar pace as cells never treated with CDDP.

Number of Cells (× 105)
Hours	OV2008 (Untreated)	OV2008 (CDDP pretreated)
0	2.28 ± 0.16	1.83 ± 0.17
48	3.98 ± 0.37	3.88 ± 0.48
72	8.08 ± 0.57	7.58 ± 0.32

Cells were treated for 1 h with 20 µM CDDP every 12 days for a total of 3 rounds on days 0, 12, and 24. Cells that were adherent on day 42 were trypsinized and replated in CDDP-free media. Once seeding was completed, the number of cells was assessed after 0, 48 or 72 h of culture. The growth of CDDP-pretreated cells was compared against the growth of an equal number of cells that never received CDDP and that were cultured in parallel under similar conditions. At the end of the incubation, cells were trypsinized and counted in a microcytometer. Data are expressed as the mean ± SEM of triplicate cultures.