Table 2.
Effect of MF on cell cycle kinetics in OV2008 cells repopulating after CDDP exposure.
| Untreated | MF | CDDP | CDDP + MF | MF | CDDP | CDDP + MF | |
|---|---|---|---|---|---|---|---|
| Day 4 |
Day 8 |
||||||
| % Sub-G1 | 2.03 ± 0.14 | 2.23 ± 0.18 | 52.3 ± 1.67* | 20.6 ± 1.45*† | 15.7 ± 4.93* | 48.7 ± 1.58* | 19.8 ± 1.56*† |
| % G1 | 56.1 ± 1.72 | 66.1 ± 0.92* | 3.22 ± 0.37* | 48.8 ± 1.32*† | 65.1 ± 2.46* | 17.1 ± 1.89*# | 7.98 ± 1.60*† |
| % S + G2/M | 33.5 ± 1.21 | 25.3 ± 0.90* | 21.6 ± 1.67* | 21.8 ± 1.61* | 14.8 ± 0.79* | 19.5 ± 1.31* | 53.3 ± 1.73*† |
| % > 4N | 8.13 ± 0.61 | 6.40 ± 1.04 | 22.9 ± 1.44* | 8.95 ± 0.86 | 4.43 ± 2.15 | 14.7 ± 0.81* | 21.6 ± 2.05*† |
Cells were treated with vehicle (0.9 % NaCl) or 20 µM CDDP for 1 h. The treatment was removed and fresh media without (untreated) or with 20 20 µM MF was replaced every 2 days. Four or 8 days after CDDP exposure, the cells were analyzed for the capacity of their DNA to bind propidium iodide by microcytometric analysis. In the groups receiving CDDP + MF we were unable to discriminate with certainty the cells that uptake propidium iodide with intensity consistent with DNA content pertaining the S or the G2/M phases of the cell cycle. Consequently, the two cell populations were measured as a whole in all experimental groups to allow comparisons. Data are mean ± SEM (n = 6, except for the group receiving MF alone with n = 3).
P < 0.05 compared to untreated
P < 0.05 compared to the time-matched CDDP.
P < 0.05 compared to CDDP on day 4. The experiment was repeated twice with similar outcomes.