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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Clin Cancer Res. 2008 Nov 1;14(21):6790–6796. doi: 10.1158/1078-0432.CCR-08-1013

Figure 2. Suppression of EZH2 inhibits pancreatic cancer cell proliferation.

Figure 2

MIA-PaCa2 and Panc04.03 pancreatic cancer cells were transfected with a control vector (shVector) or the shEZH2 silencing vector (shEZH2). A, 48 hours post-transfection, the cell pellet was collected and protein was obtained. Cell lysates were separated by SDS-PAGE, transferred to PVDF membrane, and probed with antibodies to indicated proteins. B, Relative cell viability was measured using colorimetric MTS assay at indicated times as described (13). C, Numbers of viable cells were counted by the trypan blue exclusion assay as described (30).