Fig. 4.
The multiple-inducer DIC 3-Counter construct design and results. (A) The multiple-inducer DIC 3-Counter is similar to the single-inducer DIC 3-Counter in Fig. 3 except that each promoter is a unique inducible promoter: PLtet0–1, PBAD, and PA1lacO. These promoters respond to anhydrotetracycline (aTc), arabinose, and IPTG, respectively. (B) Mean fluorescence of multiple-inducer DIC 3-Counter cell populations over time, measured by a flow cytometer. Colored areas represent the durations of consecutive inducer pulses. (C) Flow cytometry population data showing the multiple-inducer DIC 3-Counter when exposed to the desired sequence of three inducers and to single inducers only. (D) Flow cytometry population data showing the multiple-inducer DIC 3-Counter when exposed to the desired sequence of three inducers and to all pairwise permutations of inducers.