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. 2009 Jun 11;4(6):e5885. doi: 10.1371/journal.pone.0005885

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Skoglund et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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H. pylori strain HPAG1 was used for a one-year colonization of germ-free tox176 and non-transgenic (wildtype) mice with high and low gastric pH, respectively. LPS was extracted from five single-colony re-isolates from tox176 mice (n = 2) and five single-colony re-isolates from wildtype mice (n = 4) grown in vitro at both pH 7 and pH 5. Immunoblot analysis with antibodies detecting Le^x and Le^y showed that isolates from wildtype mice had switched Lewis phenotype to simultaneous Le^x and Le^y expression, as compared to the inoculating HPAG1 strain and the tox176-passaged HPAG1 isolates. Some isolates (wt3∶1, wt3∶2 and wt4∶1) appeared to express O-antigen chains that were less fucosylated when grown in vitro at pH 5 as compared to growth at pH 7. DNA sequencing showed that; FutA was “on” in all isolates; FutB was “on” in four tox176-passaged isolates (tox1:1-1:3 and tox2:1); and the number of heptad-repeats varied in active FutA and FutB enzymes, which is indicated below the lanes.