Fig. 1.
Schematic representation of the hybrid constructs used. All fusions were constructed as described in “Materials and methods”. For Sec-dependent export, AldO was fused to the signal sequences (ss) of MalE or DsbA, resulting in Sec-AldO or SRP-AldO, respectively. For export by the Tat-system, AldO was fused to the signal sequence of TorA, yielding Tat-AldO. AldO was fused to a truncated variant of INP comprising the N- and C-terminal domains for surface display. This construct was termed INP-AldO. Expression of all constructs is driven from an arabinose-inducible PBAD promotor and relevant restriction sites are indicated