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. 2009 May 12;85(5):421–431. doi: 10.1080/09553000902838566

Figure 6.

Figure 6

Cell viability of nu61 cells is reduced by neutralising antibodies to IL-6 and IL-8 and is associated with activation of apoptotic cell death. Cells were treated with either IFNγ (50 ng/ml) alone or in combination with IR (6 Gy) (see Methods). The antibodies to IL-6 and IL-8 together, but not alone, reduced viability of nu61 in response to both IFNγ alone and in combination with IR, with significance at p < 0.001 (panel A). Data on panel A are normalised to the cells treated by 50 ng/ml of IFNγ, but not treated by neutralising Abs (indicated as no Ab on X-axis). Staining for active caspase-3 (panel B) shows that neutralising antibodies to IL-6 and IL-8 activate caspase-3 in response to IFNγ + IR (representative data from three experiments). Black bars on the panel 5A represent nu61 cells treated by IFNγ and combinations of neutralising Abs to IL6 and IL8. Grey bars represent the same cells treated by the combination of IR and IFNγ. Error bars are standard deviations (n = 3) and asterisks indicate pairs with p values < 0.05.