Figure 2. The cAMP effect and glucose starvation.
A. Suppression of the cyaA mutant with exogenous cAMP. Retrohoming (RH) frequency of the MC1061-dedE::HS (WT), MC1061-cyaA-dedE::HS (cyaA), MC1061-crp-dedE::HS (crp) and MC1061-cyaA-crp-dedE::HS (cyaA crp) strains was measured in the presence of exogenous cAMP (n ≥ 3; standard deviations shown as error bars). B. Effect of glucose starvation on retromobility. Retrohoming in BL21(λDE3)-dedE::HS and retrotransposition (RTP) in BL21(λDE3) were measured with (+) and without (−) glucose as carbon source and expressed as the ratio of −Glu/+Glu (boxed), with P = 0.0027 for RTP, and P = 0.048 for RH (two-tailed paired T-test); the standard deviations are shown as error bars. The P = 0.036 for the difference between fold-increases of 5.8 for RTP and 2.6 for RH (two-tailed independent T-test).