Figure 4.

U-net components and their relative importance to the pathway and to the proteome. The figure illustrates a rank plot that reveals the presence of components of crucial importance for the U-net-specific interactions (for example, proteasome structural subunits) but not quantitatively relevant to its interaction with the proteome. On the other hand, there are other key proteins with many connections to the proteome (Ubp10 and Mpe1), but not with other Ub/Ubl pathway components. In addition, there are proteins relevant in both contexts (for example, Ubi4, Smt3, Rsp5, Rpn10), as well as proteins with just a few connections in both contexts. Gray quadrants were arbitrarily set to inspect the most important proteins in terms of degree. Essential genes are represented in bold-italic [40]. Color code: blue, proteasome components; green, Ubls; purple, F-box proteins; salmon, E1s; dark cyan, E2s; red, E3s; magenta, DUBs; dark green, others; orange, POZ; saddle brown, APC; yellow, signalosome; light blue, cullins.