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. 2009 Apr 15;16(6):859–865. doi: 10.1128/CVI.00033-09

FIG. 1.

FIG. 1.

FOF is not cytotoxic to A549 cells and does not influence RSV replication. (A) A549 cells were incubated with FOF (10 to 1,000 μg/ml) for 24 h, and their viabilities were measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell viability under cultivation without FOF was used as a control (100%; white bar). Each bar represents the mean ± standard deviation for three samples. (B) Microscopic observation of A549 cells infected or not infected with RSV (Long strain at an MOI of 1) for 24 h in the presence of FOF (0 to 1,000 μg/ml). (C) Effects of FOF on viral titers in culture supernatants and expression of RSV glycoprotein mRNA (RSV/G) in RSV-infected A549 cells. Each bar represents the mean ± standard deviation for three samples.