FIG. 1.

N. caninum-specific antibody responses of mice vaccinated with Man3-coated liposomes. Five mice per group were injected subcutaneously with PBS, NcGRA7t-GST in PBS (NcGRA7), NcGRA7t-GST entrapped within Man3-coated liposomes (M3-NcGRA7), or GST entrapped within Man3-coated liposomes (M3-GST) at days 0, 7, and 14. ELISA using NLA measured the parasite-specific IgG1 and IgG2a antibodies in sera. The values are expressed as optical densities at 415 nm. Each bar represents the mean ± standard deviation (SD) for five mice per group. Results are representative of two independent experiments. *, statistically significant differences were observed between the two groups immunized with NcGRA7 and M3-NcGRA7 by Student's t test on the same day after immunization (P < 0.01).