FIG. 2.

Production of IFN-γ from spleen cells. Three mice per group were injected subcutaneously with PBS, NcGRA7t-GST in PBS (NcGRA7), NcGRA7t-GST entrapped within Man3-coated liposomes (M3-NcGRA7), or GST entrapped within Man3-coated liposomes (M3-GST) at days 0, 7, and 14 and then sacrificed at day 21. Single-cell suspensions were prepared from the spleens of individual mice in the groups and cultured for 48 h in the presence of 50 μg/ml NLA, 10 μg/ml NcAMA1, or 10 μg/ml NcGRA7 or without any stimulator (medium). Culture supernatants were assayed for IFN-γ production by ELISA. Each bar represents the mean ± SD for three mice per group. Results are representative of two independent experiments. *, statistically significant differences were observed between the two groups immunized with NcGRA7 and M3-NcGRA7 by Student's t test on the same day of immunization (P < 0.01).