FIG. 3.
Survival rates of offspring in trials 1 and 2. Female mice were immunized subcutaneously with NcGRA7t-GST entrapped within Man3-coated liposomes (M3-NcGRA7), GST entrapped within Man3-coated liposomes (M3-GST), NcGRA7t-GST in PBS (NcGRA7), or PBS. Booster immunizations were administered 7 and 14 days after the first immunization. Seven days after the third immunization, the mice were housed with males for 3 days. All pregnant dams were challenged on the same day with 1 × 105 tachyzoites, at 6 to 9 days of gestation. Numbers of offspring were monitored for 30 days after birth.