TABLE 1.
Survival rates of offspringa
| Group | Trial | No. of litters | Mean no. of offspring/litter (SD) | No. of surviving offspring/no. of offspring in litter | Total no. of surviving offspring/total no. of offspring (%) |
|---|---|---|---|---|---|
| PBS | 1 | 4 | 6.8 (2.6) | 2/3, 2/7, 0/8, 2/9 | 6/27 (22.2) |
| 2 | 5 | 7.2 (2.2) | 1/5, 0/8, 0/10, 4/8, 0/5 | 5/36 (13.4) | |
| Total | 9 | 7.0 (2.3) | 11/63 (17.5) | ||
| M3-GST | 1 | 4 | 6.8 (1.7) | 0/7, 3/6, 1/9, 3/5 | 7/27 (25.9) |
| 2 | 5 | 6.4 (1.3) | 0/7, 3/5, 0/7, 1/8, 0/5 | 4/32 (12.5) | |
| Total | 9 | 6.6 (1.4) | 11/59 (18.6) | ||
| NcGRA7 | 2 | 4 | 6.3 (2.6) | 2/8, 0/9, 1/4, 0/4 | 3/25 (12.0) |
| M3-NcGRA7 | 1 | 4 | 6.5 (1.3) | 5/7, 8/8, 3/6, 3/5 | 19/26 (73.1) |
| 2 | 5 | 5.0 (2.6) | 2/4, 0/2, 5/6, 7/9, 2/4 | 16/25 (64.0) | |
| Total | 9 | 5.7 (2.2) | 35/51 (68.6)* |
a
Female mice were immunized subcutaneously with NcGRA7t-GST entrapped within Man3-coated liposomes (M3-NcGRA7), GST entrapped within Man3-coated liposomes (M3-GST), NcGRA7t-GST in PBS (NcGRA7), or PBS. Booster immunizations were administered 7 and 14 days after the first immunization. Seven days after the third immunization, the mice were housed with males for 3 days. All pregnant dams were challenged on the same day with 1 × 105 Nc-1 tachyzoites, at 6 to 9 days of gestation. For the survival rates of offspring at 30 days after birth, statistical differences versus the PBS group were determined by χ2 test (*, P < 0.01).