TABLE 2.
PCR detection of N. caninum in damsa
| Group | Trial | No. of positive dams/total no. of dams (%) |
|---|---|---|
| PBS | 1 | 4/4 (100) |
| 2 | 5/5 (100) | |
| Total | 9/9 (100) | |
| M3-GST | 1 | 4/4 (100) |
| 2 | 5/5 (100) | |
| Total | 9/9 (100) | |
| NcGRA7 | 2 | 4/4 (100) |
| M3-NcGRA7 | 1 | 1/4 (25) |
| 2 | 2/5 (40) | |
| Total | 3/9 (33.3)* |
a
Female mice were immunized subcutaneously with NcGRA7t-GST entrapped within Man3-coated liposomes (M3-NcGRA7), GST entrapped within Man3-coated liposomes (M3-GST), NcGRA7tGST in PBS (NcGRA7), or PBS. Booster immunizations were administered 7 and 14 days after the first immunization. Seven days after the third immunization, the mice were housed with males for 3 days. All pregnant dams were challenged on the same day with 1 × 105 Nc-1 tachyzoites, at 6 to 9 days of gestation. DNAs were extracted from the brains of dams 40 days after infection and were then analyzed by PCR. Statistical differences versus the PBS group were determined by χ2 test (*, P < 0.01).