FIG. 4.

Western blot of C. trachomatis-positive pediatric sera. Sera with Pgp3 assay absorbance values above 0.2 were assayed by Western blotting. Lysed C. trachomatis EB proteins were separated on a 12.5% polyacrylamide gel and then transferred to a PVDF membrane. Pediatric sera (n = 75) diluted 1:100 were assayed, and those considered positive (>8 bands reactive) are shown. A positive-control serum sample (C) is included. Common C. trachomatis immunodominant antigens are shown, including the several ∼99-kDa polymorphic membrane proteins (Pmps), the 72-kDa hsp70, the 71-kDa outer membrane protein 2 (OMP2), the 61-kDa hsp60, MOMP, and the macrophage infectivity potentiator (MIP) protein.