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ROC curves for 356 C. trachomatis-positive sera and either 740 pediatric sera (after exclusion of sera that were positive for EB by Western blotting) (A) or 722 pediatric sera (after the additional exclusion of sera that were positive for C. trachomatis by MIF) (B). The broken line identifies the point on the ROC curve in panel A corresponding to the absorbance at 450 nm, chosen as the final cutoff for the assay.
