Skip to main content
. 2009 Apr 22;16(6):906–915. doi: 10.1128/CVI.00413-08

FIG. 1.

FIG. 1.

Determination of antibody and conjugate reactivity against sera from different odontocete species. (A) Rabbit antiserum anti-IgG from S. bredanensis (S) was tested by immunodiffusion against sera from Delphinus delphis (Dd), Feresa attenuata (Fa), Grampus griseus (Gg), Kogia breviceps (Kb), Kogia sima (Ks), Lagenodelphis hosei (Lh), Mesoplodon europaeus (Me), Orcinus orca (Oo), Peponocephala electra (Pe), Physeter macrocephalus (Pm), Stenella attenuata (Sa), Stenella clymene (Scl), Stenella coeruleoalba (Sc), Stenella frontalis (Sf), Stenella longirostris (Sl), Steno bredanensis (Stb), and Tursiops truncatus (Tt). Serum from Bos taurus (Bt) was used as an out-group control. (B) An anti-S. bredanensis IgG-HRP conjugate was tested by WB against sera from 17 different odontocete species and a cow, and the reactivity against the heavy-chain IgG was measured by pixel densitometry. The WB experiments were performed at least four times, and the density of the IgG heavy-chain band of each serum species normalized using the IgG-heavy chain of S. bredanensis. Notice the weaker and partial reaction of Kogia species in immunodiffusion and the significantly weaker reaction of these species in WBs. *, P < 0.05; **, P < 0.005.