TABLE 1.
Mutagenic primers used to repair base errors in TES-26 recombinant plasmids
| Mutagenic primer | Sequence (5′ to 3′) (25-mer) | Purpose |
|---|---|---|
| TES26MF1 | TTCACACGACCAGTCAGCCAAGTTC | G to replace A |
| TES26MR1 | GAACTTGGCTGACTGGTCGTGTGAA | G to replace A |
| TES26MF2 | AGCGCCGCGAACGGCCAGCAGGGTC | G to replace C |
| TES26MR2 | GACCCTGCTGGCCGTTCGCGGCGCT | G to replace C |
| TES26MF3 | CCGCCGCTAATACTGGTGTGCACCG | C to replace G |
| TES26MR3 | CGGTGCACACCAGTATTAGCGGCGG | C to replace G |
| TES26MF4 | TATGCTGGCAATTTCTATCGATCGC | T to replace C |
| TES26MR4 | GCGATCGATAGAAATTGCCAGCATA | T to replace C |