TABLE 1.
Resolution of discrepancies generated by MRSA PCR from incubation of 322 clinical nasal swab specimen lysates in freshly reconstituted master mix or experimental frozen master mix stored at −70°C
| Specimen no. | PCR result for master mix
|
Final interpretation | |||
|---|---|---|---|---|---|
| Initial
|
Repeat
|
||||
| Frozen | Freshly reconstituted | Frozen | Freshly reconstituted | ||
| 1 | Unresolved | − | Unresolved | − | Unresolved result from frozen master mix |
| 2 | + | − | + | − | Confirmation of discrepancies |
| 3 | − | + | − | + | Confirmation of discrepancies |
| 4 | − | + | − | + | Confirmation of discrepancies |
| 5 | − | + | + | − | Unable to replicate either initial PCR result; exclusion from data analysis |
| 6 | − | + | + | − | Unable to replicate either initial PCR result; exclusion from data analysis |
| 7 | − | + | − | − | Unable to replicate initial PCR result from freshly reconstituted master mix |
| 8 | − | + | − | − | Unable to replicate initial PCR result from freshly reconstituted master mix |
| 9 | − | + | − | − | Unable to replicate initial PCR result from freshly reconstituted master mix |
| 10 | − | + | − | − | Unable to replicate initial PCR result from freshly reconstituted master mix |
| 11 | − | + | − | − | Unable to replicate initial PCR result from freshly reconstituted master mix |
| 12 | − | + | − | − | Unable to replicate initial PCR result from freshly reconstituted master mix |