TABLE 4.
Hypothetical matrix relating lysate batch size to the potential for discard of freshly reconstituted master mix under current BD GeneOhm MRSA assay specifications
| Lysate batch size (size with controls included) | No. (%) of unused aliquots following reconstitutiona
|
||
|---|---|---|---|
| 8 aliquots/vial | 9 aliquots/vial | 10 aliquots/vial | |
| 1 (3) | 5 (62.5) | 6 (66.7) | 7 (70.0) |
| 2 (4) | 4 (50.0) | 5 (55.6) | 6 (60.0) |
| 3 (5) | 3 (37.5) | 4 (44.4) | 5 (50.0) |
| 4 (6) | 2 (25.0) | 3 (33.3) | 4 (40.0) |
| 5 (7) | 1 (12.5) | 2 (22.2) | 3 (30.0) |
| 6 (8) | 0 (0.0) | 1 (11.1) | 2 (20.0) |
| 7 (9) | 7 (43.8) | 0 (0.0) | 1 (10.0) |
| 8 (10) | 6 (37.5) | 8 (44.4) | 0 (0.0) |
| 9 (11) | 5 (31.3) | 7 (38.9) | 9 (45.0) |
| 10 (12) | 4 (25.0) | 6 (33.3) | 8 (40.0) |
| 11 (13) | 3 (18.8) | 5 (27.8) | 7 (35.0) |
| 12 (14) | 2 (12.5) | 4 (22.2) | 6 (30.0) |
| 13 (15) | 1 (6.3) | 3 (16.7) | 5 (25.0) |
| 14 (16) | 0 (0.0) | 2 (11.1) | 4 (20.0) |
a
Mandated controls were added to the batch size prior to the calculation of unused reaction tubes. The matrix assumes the presence of one 16-thermocycler block in a laboratory. The mean percentages of unused aliquots per random distribution batch size were 24.4, 29.6, and 33.5%, respectively, for 8, 9, and 10 aliquots per master mix vial.