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. 2009 Apr 22;47(6):1757–1766. doi: 10.1128/JCM.02019-08

FIG. 1.

FIG. 1.

FIG. 1.

Gel electrophoresis and FLA of 15 VNTR loci from five Cebu, the Philippines, clinical isolates amplified using the combination 1, 2, 3, and 4 primer sets. (Aa, Ba, Ca, and Da) Gel electrophoresis of multiplex-PCR products (combinations 1 to 4). Lanes: M, 20-bp DNA marker (Bio-Rad); P, multiplex-PCR products from NHDP63 (positive control); 28, 32, 35, 39, and 42, multiplex-PCR products from clinical isolates; N, negative control. (Ab, Bb, Cb, and Db) Electropherograms obtained from FLA, where the orange peaks represent the LIZ-500 size standards and red, blue, black, and green peaks represent the VNTR products in each of the combinations. Arrows indicate the peaks selected as the allele for each locus.