TABLE 1.

Multiplex STR primers in four different combinations

Multiplex PCR combination no.	Locusa	Forward primerd	Tm (°C)e	Reverse primer	Tm (°C)	Amplicon sizeb (bp)
1	(AC)8b	VIC-GCCCACTTACCTCAACCAAC	65.4	CCTATAACGGCACTCAGTCCA	61.4	390
	(GTA)9	NED-AGCCTTAGTCGCGCAGATG	62.6	TCCGCTGTCCGTCCGCTGA	68.8	307
	(GGT)5	6FAM-GCAGCGGTGTAACAGCATAGC	68.4	TGTCTGCCTTGCGAAACGGTC	65.5	242
	(AT)17	PET-TCTCCAACATGCTGCGACA	62.1	GTACAGCGGCCTGATCGAA	60.0	181
	6-3ac (rpoT)	VIC-ATGCCGAACCGGACCTCGACGTTGA	60.0	TCGTCTTCGAGGTCGTCGAGA	59.5	91
2	21-3	6FAM-GAATCTGACCTTTCGGAAATG	56.8	CGATGCAGCTTCCTACGG	60.7	312
	(AC)9	NED-AGCGCCCGTTGTCGATAGA	63.9	GACTGGATGTCGGCACCCC	65.5	236
	(AT)15	PET-CAATATGCGGGTTGGCGCTTCTG	66.2	CCGTCTGGCTCGATGGCTGGATTC	68.6	168
	(AC)8ac	VIC-GTGTTACGCGGAACCAGGCA	65.5	CCATCTGTTGGTACTACTGA	53.5	124
3	27-5	6FAM-ATTGAGCAGATGGCCGGTC	62.8	AGCAGTCGGCACGCCCTT	67.8	327
	6-7	VIC-GCCATCGTTGTCGGTTCATC	61.5	CGGAGGAGGTGGGTACGGT	66.1	268
	(TA)18	NED-CGTGCGTCGTGTGTAGGC	63.9	GACGTGGCAACATCGAAGTT	61.0	230
	(GAA)21	PET-CTACAGGGGGCACTTAGCTC	62.1	GGACCTAAACCATCCCGTTTT	60.4	201
4	18-8	PET-GCCCGTCTATCCGCATCAA	62.5	GCAAAGATCAGCACGCCAAT	61.8	348
	12-5	VIC-CTGGTCCACTTGCGGTACGAC	65.1	GGAGAAGGAGGCCGAATACA	61.4	289
	23-3	6FAM-CCGAAGCCCTGGACGAAG	63.1	GCCGTAAATCCGCTCCC	56.0	243
	(TA)10c	PET-TAGATTCAAACGACCATGCA	60.0	TGATAATCACGTGTTTCCGC	60.0	185

^aTandem repeat locus nomenclature per Groathouse et al. (6).

^bAmplicon sizes per the sequenced M. leprae TN strain (4).

^cPrimer sequences per Zhang et al. (34).

^dVIC, NED, 6FAM (6-carboxyfluorescein), and PET refer to the 5′ fluorochrome.

^eT_m, melting temperature.