TABLE 2.
Specificities and sensitivities of different real-time PCR assays on rhinovirus and enterovirus plasmid-derived constructs
| Targeta | Results from indicated assaysb
|
|||||
|---|---|---|---|---|---|---|
| RHP1 | RHP2 | Panenterhino | Panenterhino/Ge/08 | HEV | Entero/Ge/08 | |
| HRV-2 | 34.7 (±0.2) | Neg | 34.2 (±0.2) | 34.5 (±0.2) | Neg | Neg |
| HRV-14 | Neg | 42.7 (±2.1) | 34.3 (±0.5) | 35.3 (±1.3) | Neg | 36.3 (±2.3) |
| HRV-C | Neg | Neg | 34.9 (±0.1) | 34.7 (±0.1) | Neg | Neg |
| HRV-C′ | Neg | Neg | 46.5 (±6.5) | 37.4 (±0.7) | Neg | Neg |
| EV-71 | Neg | Neg | 35.7 (±0.2) | 35.6 (±0.4) | 33.9 (±0.3) | 31.9 (±0.2) |
| EV-104 | Neg | Neg | 34.7 (±0.2) | 34.6 (±0.2) | Neg | 35.2 (±0.1) |
a
HRV- and HEV-derived plasmids were used at a uniform concentration of 1,000 copies of input per PCR. Plasmid constructs are described in Materials and Methods. Results are indicated as real-time PCR CT values (± standard deviations).
b
Neg, negative result.