TABLE 2.
Comparison of results obtained by PNA array, conventional direct sequencing, and specific PCRa
| PNA array | Identification by:
|
|
|---|---|---|
| Sequencing | Specific PCR | |
| 6 (15) | 6 (15) | -c |
| 11 (5) | 11 (5) | - |
| 16 (6) | 16 (6) | - |
| 18 (5) | 18 (5) | - |
| 31 (1) | 31 (1) | - |
| 35 (1) | 35 (1) | - |
| 53 (5) | 53 (5) | - |
| 61 (0)b | 61 (1) | - |
| 62 (1) | 62 (1) | - |
| 66 (3) | 66 (3) | - |
| 70 (2) | 70 (2) | - |
| 81 (3) | 81 (3) | - |
| 83 (1) | 83 (1) | - |
| 84 (0)b | 84 (1) | - |
| 11,45d | 11 | 11,45 |
| 53,70d | 53 | 53,70 |
| 53,56d | 53 | 53,56 |
| 18,44d | 18 | 18,44 |
| 53,35d | 53 | 53,35 |
| 62,58d | 62 | 62,58 |
| 31,53d | 31 | 31,53 |
| 6,39d | 6 | 6,39 |
| 53,66,70d | 66 | 53,66,70 |
| 16,53 | 16,53 | - |
| 16,53,54d | 16,53 | 16,53,54 |
a
We used three approaches to identify the clinical samples. The numbers are HPV types. The numbers in parentheses are the numbers of the types detected. Total, 72; negative, 12; positive, 60.
b
Probes for HPV types 61 and 84 were not included in the PNA array.
c
Type-specific PCR was not performed in cases where PNA array results and sequencing results were in good agreement.
d
These 10 case of multiple infections were confirmed to have mixed infection on analysis by type-specific PCR and sequencing.