TABLE 2.
Oligonucleotide primers used for sPCR
| Primer name | Specificity (target) | GenBank accession no. | Primer sequencea (5′-3′) | Tmb (°C) | Reference |
|---|---|---|---|---|---|
| Tv1 | T. vaginalis 18S rRNA gene | U17510 | 874TAA TGG CAG AAT CTT TGG AG894 | 59.2 | 25 |
| Tv2 | 1185GAA CTT TAA CCG AAG GAC TTC1165 | 58.3 | |||
| HSVPolA1 | HSV DNA polymerase | ATC ATC TAC CGC GAC ACG GACT | 68.8 | 49 | |
| HSVPolA2 | TCC ACG CCC TTG ATG AGC ATC T | 72.0 | |||
| MG16-45F | M. genitalium 16S rRNA gene | X77334 | 45TAC ATG CAA GTC GAT CGG AAG TAG C69 | 68.8 | 16 |
| MG16-447R | 469AAA CTC CAG CCA TTG CCT GCT AG447 | 69.2 | |||
| AD1 | Adenovirus hexon gene | U20821 | CTG ATG TAC TAC AAC AGC ACT GGC AAC ATG GG | 76.1 | 36 |
| AD2 | GCG TTG CGG TGG TGG TTA AAT GGG TTT ACG TTG TCC AT | 83.4 | |||
| GV1 | G. vaginalis | LO8167 | 364TTA CTG GTG TAT CAC TGT AAG G385 | 55.8 | 51 |
| 16S-23S rRNA gene | |||||
| GV2 | 23S | 695CCG TCA CAG GCT GAA CAG T677 | 64.1 |
a
Numbers represent the base positions at which the primer/probe sequence starts and finishes (starting at point 1 of the corresponding gene sequence in GenBank).
b
Melting temperatures were provided by the primer synthesizer (Sigma-Aldrich).