TABLE 1.
Primer sequences used for six VNTR target loci from Salmonella enterica serovar Newport
| Target | Forward primera | Reverse primer | Repeat size (bp)b | Amplicon size (bp) |
|---|---|---|---|---|
| PCR 1 | ||||
| STTR6 | 5′-FAM-TCGGGCATGCGTTGAAA | 5′-CTGGTGGGGAGAATGACTGG | 6 | 397 |
| Newport-A | 5′-PET-ACTGAAAGGAAGGGGAGAGC | 5′-GTCAGGGTGGAATAGAATGC | 9 | 429 |
| Newport-L | 5′-VIC-GAAGTACCGAAGTGGGTGAT | 5′-CGTCCGTTAGAGGAACGTAT | 51 | 529 |
| PCR 2 | ||||
| STTR5 | 5′-PET-ATGGCGAGGCGAGCAGCAGT | 5′-GGTCAGGCCGAATAGCAGGAT | 6 | 564 |
| Newport-B | 5′-VIC-GGCCGATATAGCTCAGTTGG | 5′-GAACCTCGCTTAGGGTTGTG | 12 | 350 |
| Newport-M | 5′-FAM-GGTCATAGAGGGTCTGCAT | 5′-ATGGAGCACAGACCACTAAC | 36 | 378 |
a
6-Carboxyfluorescein (FAM), PET, and VIC are 5′-conjugated fluorescent dyes (Applied Biosystems).
b
Expected repeat size for Salmonella enterica serovar Newport strain SL254 (GenBank accession number NC_011080).