Fig. 2.
p27 and β-catenin (B-cat) expression in Wnt-1 tumors. (A) p27 and B-cat staining of normal breast and breast tumor from Wnt-1 mice. Arrows point to positive cells. Left column photographs taken at ×100 and right column at ×600 magnification. (B) Comparison of B-cat staining in breast and intestinal (gastrointestinal) tumors from ApcMin mice and Wnt-1 transgenic mice. Middle row photographs taken at ×100 and bottom row at ×600 magnification. (C) Immunoblot analysis of p27 and cyclin D1 in Wnt-1 breast tumors from the following mice. Lanes 1–3: intact female; lanes 4–6: E- and P-treated female; lanes 7–9: male; lane 10: p27 null; lane 11 and12: normal breast. n, nuclear fraction and c, cytoplasmic fraction.