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. Author manuscript; available in PMC: 2010 Jun 10.
Published in final edited form as: J Comp Neurol. 2009 Jun 10;514(5):449–458. doi: 10.1002/cne.22016

Figure 1.

Figure 1

NT-3 expression in retinas of wild type (WT) and NT-3 overexpressing (NT-3 OE) mice. (A) A representative Western blot analysis for NT-3 on retinal protein extracts isolated at different postnatal ages. GAPDH was used as an internal control of the loading. (B) Quantification of relative retinal NT-3 protein levels shows that dark rearing (DR) did not change NT-3 expression level compared to mice reared in 12hr dark/12hr light cycles (Normal Rearing, NR). A representative Western blot for NT-3 and GAPDH is inserted on the top. (C–F) Confocal fluorescence micrograph of WT mouse retina double immunostained with antibodies against NT-3 (magenta), and Brn-3a, CaBP5, calbindin and TH (green), respectively. (G–H) Confocal fluorescence micrographs of immunolabeling of NT-3 (G) and CaBP5 (H) in WT and NT-3 OE retinas. ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar: 20 μm. (I) The IPL thickness increased from P13 to P28 in the WT retina and overexpression of NT-3 did not change the thickness of the IPL at P13 and P28. *:P < 0.05 in Student’s t-test.