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. 2009 May 13;420(Pt 2):221–227. doi: 10.1042/BJ20090110

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© 2009 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

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The increase in O-GlcNAc levels in comparison with untreated samples is shown in the histogram underneath the blot. (A) HEK-293 cells were treated with GlcNAcstatins A–E for 6 h with the concentrations indicated. (B) GlcNAcstatin C was added to HeLa, HT-1080, SH-SY5Y or U-2 OS cells for 6 h with the identical inhibitor concentrations as in (A). The molecular mass in kDa is indicated on the left-hand side of each blot.