Figure 1.

Methodological procedures to quantify the mitochondrial concentration in each suspension. (A) The number of mitochondria per micrometer was assessed by counting the mitochondria that were situated under the four lines crossing the center of the micrograph; the final number of mitochondria per micrometer was established as the mean value of the four counts. The number of mitochondria per square meter was evaluated by counting the total mitochondria present in each micrograph. (B) Assessment of the correction factor for centrifugation-induced compaction, in order to adjust the data drawn from the microscopic evaluation of the pellet to the real volume of the mitochondrial suspension.